Synchronization of Somatic Embryogenesis in a Carrot Cell Suspension Culture

Synchronization of somatic embryogenesis in a carrot cell suspension culture.

Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were compose...

Carrot Suspension Culture Cells

Somatic embryogenesis in Phoenix dactylifera: maturation, germination and reduction of hyperhydricity during embryogenic cell suspension culture

This work describes an improved and efficient method for optimum maturation and normal embryo growth to produce healthy plantlets through somatic embryogenesis using suspension cultures for date palm cv. sakkoty. cell suspensions were supplemented with low amounts of dichlorophenoxyacetic acid (0.5mg/1) and different concentrations of abscise acid (AbA) (0.1, 0.3 and 0.5 mg/l) and polyethylene ...

Cell Suspension Culture, Somatic Embryo Genetic and Synthetic Seed Production in Different Cultivars of Potato

Extended Abstract Introduction and Objective: In order to overcome the contamination problems, as well as the usual method of increasing potato through micro tuber, micropropagation has been considered through tissue culture. The aim of this study was to evaluate the callus induction and embryogenesis in potato cultivars using leaf explants. Material and Methods: For this purpose, micropropa...

Identification of a transitional cell state in the developmental pathway to carrot somatic embryogenesis

We have located a novel carbohydrate epitope in the cell walls of certain single cells in embryogenic, but not in non-embryogenic, suspension cultures of carrot. Expression of this epitope, recognized by the mAb JIM8, is regulated during initiation, proliferation, and prolonged growth of suspension cultures such that changes in the abundance of JIM8-reactive cells always precede equivalent chan...